Santacruz Biotechnology Inc
p-Erk1/2 (Thr202/Tyr 204)
sc-16982
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The aim of the study was to detect the effect of gene knockdown on growth factor induced Erk1/2 phosphorylation in mouse pancreatic endothelial cells (MPECs). Cells were transfected with specific siRNA, and after 48 hrs growth arrested, treated with growth factor, protein were extracted, equal amount resolved in 10% SDS-PAGE, and immunoblotted with specific p-Erk1/2 antibody procured from SantaCruz Biotechnology. Selection of antibody was based on information’s available in antibody flayer.
Western Blot
Mouse pancreatic endothelial cells
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 2hr at RT
None
Enhanced chemiluminiscent detection in X-Ray films
p-Erk1/2 antibody is an excellent antibody gives only specific band at desired molecular weight.
Highly specific antibody
Excellent antibody