Chicken IFN-gamma Cyto-Set - IFN-gamma Determination in Chicken Tissues

May 24, 2016

UFPR
Patologia Básica
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Quality of Results

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Company:

Life technologies

Product Name:

Novex - Chicken IFNγ Cyto-set

Catalog Number:

CAC1233

We are interested in the IFNy levels in chicken tissues like the intestine. We chose this assay kit because it has a good proportion of cost vs benefit.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Samples of chicken tissues

Protocol Overview

We take the protocol from the lot-specific technical data sheet.

Tips

It's appropriate to standardize the method for the specific tissue before the real experiment.

Results Summary

The results obtained are very replicables, and the spectrum of determination of IFNy are between 62,5 and 1500 pg/mL.

Additional Notes

Sometimes, the mininum IFNy cannot be determinated

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Summary

The Good

The protocol is very practical

The Bad

The protocol does not specify the amount of tissue that is required.

The Bottom Line

This product works well but because of the lack of informations in the technical data sheet, we needed to spend a lot of time and reagents/tissues in the standardization.

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