Cell Signaling Technology
P-S6(235/236)
4858S
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This rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. The primary antibody dilution is 1:1000 in 5% BSA.
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA
1:10000 dilution of anti-rabbit secondary antibody, 1hour at room temperature
NA
ECL
The band is very strong and clear no matter what kind of ECL substrate is used for exposure, and is increased in the tumor tissues.
None
A good antibody
It's a very good antibody with high quality.