The Good
Directly conjugated with a fluorophore. Minimal optimization. Bright staining of cells. Works with both human and mouse (reported by manufacturer).
The Bad
Fixing and permeabilization of cells required prior to antibody incubation, resulting in a longer cell staining procedure.
The Bottom Line
This antibody performs very well in flow cytometry and is comparable to other anti-CD3 antibodies. If you work with both human and mouse samples, you may want to consider this product over other species specific monoclonals. If fixing and permeabilizing your samples is not an option, you may want to consider a different antibody. Highly recommended!