Anti-MAPRE2 Antibody in Western-Blot

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

Anti-MAPRE2 antibody produced in rabbit

Catalog Number:

HPA016739 Sigma

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF7, Caco-2 cell lysates

Primary Incubation

1:1000 diluted in 1% BSA, incubation overnight

Blocking Agent

5% milk powder

Secondary Incubation

HRP coupled secondary antibody diluted 1:5000 in 1% BSA for 30 min at room temperature

Tertiary Incubation

None

Detection

ECL detection

Results Summary

Specific bands for MAPRE2

Additional Notes

None

Related Categories

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Summary

The Good

Detection of specific bands for MAPRE2

The Bad

None

The Bottom Line

Good antibody for the detection of MAPRE2 bands in Western-Blot

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