Good Staining of CD1c PE-Vio770

May 10, 2016

Department of Hematology/Oncology
MSSM
Associate Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD1c PE-Vio770

Catalog Number:

130-101-581

CD34 cells were isolated from cord blood samples and differentiated into myeloid cells. CD1c PE-Vio770 was used to identify CD1c DCs in the differentiated populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Differentiated myeloid cells from CD34 (cord blood)

Primary Incubation

1:200, 30 minutes at 4 deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (LSR Fortessa)

Results Summary

CD1c was positively identified as Lineage negative, CD1c+, HLA-DR+

Additional Notes

None

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Summary

The Good

Good antibody

The Bad

It isn't the brightest on the market.

The Bottom Line

Works well and is reproducible.

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