BD Horizon
Fixable Viability Stain 780
565388
This fixable viability stain was used to discriminate dead cells in primary murine tumor cells. Freshly isolated cells were stained with FVS780 followed by incubation with fluorochrome-conjugated antibodies. The nice feature of this product is that the cells can be fixed afterwards (if neccessary), while preserving the viability staining (unlike other conventional viability dyes).
Flow cytometry
Murine tumor cells
Frehsly isolated cells were incubated with FVS780 (1:1000) in FACS staining buffer for 15min at 4C.
Incubation with FVS780 can be performed before antibody staining (as recommended by the company) or at the same time without losing any signal
Easy discrimination of live and dead cells, with no difference in signal before or after fixation.
None
Bright, can be used on fixed and unfixed cells
No complaints
Easy to use live/dead stain, great for samples that need to be fixed (eg for intracellular staining)