Chemotaxis Slide for Suspension and Adherent Cells

March 16, 2016

LMU Munich
Anaesthesiology
Researcher

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Performance

Ease-of-Optimization

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Company:

Ibidi

Product Name:

µ slide Chemotaxis

Catalog Number:

80326

With this slide, we quantified chemotaxis of suspension cells (monocytes) using a chemoattractant. After acquiring images, the resulting movie may be quantified with Image J or a different cell tracking program.

Experimental Design and Results Summary

Application

Chemotaxis, time lapse microscopy

Starting Material

Cell suspension

Protocol Overview

According to manufacturer's instructions, use 18.00 cells/channel. For suspension cells it is recommended to mix cells with collagen I in order to form a gel in the slides. The optimal collagen I concentration must be titrated. Fill the reservoirs surrounding the channel with the desired chemoattractant or control media. Incubate for the desired time uner a time-lapse microscope acquiring image every 2 -4 minutes.

Tips

Use beveled tips wehen pipetting the cells into the channel, others will get stuck. Degas equipent in a incubator overnight (including media)

Results Summary

With this slide, you can quantify single-cell movements with a cell tracking software. First, it will be a little tricky to fill the channels with the cells, since bubbles are introduced really easily. Here, the use of beveled pipette tips will help. Also, it is crucial to titrate the correct Collagen I concentrations. Having done this, this slide provides you with great movies, since the optic properties of the slides are really good. Be sure to pipette fast since the collagen gels really quickly.

Features Summary

The reservoirs for the chemoattractant are filled easily, the instructions supplied by the manufacturer are really clear.

Additional Notes

You may need to adjust the pH of the media after introducing the acidic collagen I.

Image Gallery

Summary

The Good

Creates nice images

The Bad

Handling technique is not easy, needs to be practiced, special tips are needed.

The Bottom Line

Good method for quantifying chemotaxis of adherent and supension cells. The evaluation of the resulting pictures is a little time consuming using image J, but a special software is also available (chargeable).

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