Santa Cruz Biotechnology
Cofilin Antibosy (FL-166)
sc-33779
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Browse Similar Products: Cofilin Western Blot Antibodies
Aortic smooth muscle cell migration into tunica intima is a major cause of restenosis. We have tested various signaling molecules levels in vascular smooth muscle cells to understand the mechanism of their migration. Here, we tested the time-course effect of a chemokine on expression of cofilin in human aortic smooth muscle cells.
Western Blot
Aortic smooth muscle cell lysates
5% skim milk, 1 hr at room temperature
5% skim milk
1:500 dilution/ over night / 4 deg C
Goat anti-rabbit, 1:4000, room temperature for 1 hr
Horseradish peroxidase (HRP)
Time-course effect of chemokine has no effect on cofilin expression.
None
Very specific and no background
Needs optimization.
Good antibody and very specific