Cell Signaling Technology
Phospho-Src Family (Tyr416) Antibody
2101S
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Aortic smooth muscle cell migration into tunica intima is a major cause of restenosis. We have tested various signaling molecules levels in vascular smooth muscle cells to understand the mechanism of their migration. Here, we tested the time-course effect of a chemokine on phosphorylation of Src kinase in human aortic smooth muscle cells.
Western Blot
Aortic Smooth Muscle Cell lysates
5% skim milk, 1 hr at room temperature
5% BSA
1:1000 dilution/ over night / 4 deg C in TBST + 5% BSA
Goat anti-rabbit, 1:4000, room temperature for 1 hr
Horseradish peroxidase (HRP)
Chemokine has activated src kinases in a time-dependent manner.
None
The antibody is very good and easy to optimize
Gives non-specific bands
This antibody is good but gives a few non-specific bands.