Strong Signal Surface Staining Antibody, Some Leakage in PE Channel

Rheumatology
University of California
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

CD11c-PE cy7

Catalog Number:

117317

To study the distribution of CD11c on in vitro cultured myeloid cells we stained the cells with CD11cPEcy7 and identified rare populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

In vitro cultured cells

Primary Incubation

1:1000 for 2hrs

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Green/Yellow-green laser by flow

Results Summary

Good signal was obtained and unique populations were identified. However some leakage does occur in PE channel. So if proper gating is done, identification is possible

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Cheap and strong signal

The Bad

Tandem conjugate needs to be protected against light

The Bottom Line

Overall good specific antibody

Share your experience with other scientists. Write a Review! »

Join the discussion