Santacruz Biotechnology Inc
ADAM10 antibody (B-3)
SC-28358
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Mammalian cell were time dependently treated with growth factor, cells were lysed in RIPA buffer, proteins were extracted, equal amount of proteins were resolved in 10% SDS-PAGE, and immunoblotted with anti-ADAM10 antibody. Selection of antibody was based on information provided in antibody flyer.
Western Blot
Whole cell lysate
Dilution (1:500) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Anti-ADAM10 antibody from SantaCruz biotech has detected a specific band of un-cleaved and cleaved ADAM10 at nearly 90 kDa, and 42 kDa, respectively. No nonspecific bands were observed.
Detected both cleaved and un-cleaved of ADAM10
Nice antibody for Immunoblotting