Cell Signaling Technology
PAK1 Antibody
2602
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Smooth muscle cells from mouse pups were subjected to specific treatment, then were collected and PAK1 steady state levels were examined using Western blotting. Proteins were extracted, an equal amount of proteins were resolved in 10% SDS-PAGE, and immunoblotted with anti-PAK1 antibody procured from Cell Signaling Technology. Selection of antibody was based on information provided in antibody flyer.
Western Blot
Mouse smooth muscle extract
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
PAK1 antibody detected only specific band of PAK1 protein at desired molecular weight. However, there was effect of treatment on PAK1 steady state levels.
Gives highly specific single band
Excellent antibody