Cell Signaling Technology
Phospho-PKCtheta (Thr538) antibody
9377
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Browse Similar Products: Phospho PKC-theta Western Blot Antibodies
We tested the time-course effect of a chemokine on phosphorylation of PKC-theta in Human aortic smooth muscle cells.
Western Blot
Aortic Smooth Muscle Cell lysates
5% BSA, 1 hr at room temperature
5% BSA
1:1000 dilution/ over night / 4 deg C
Goat anti-rabbit, 1:5000, room temperature for 1 hr
Horseradish peroxidase (HRP)
We found that chemokines induce the phosphorylation of PKCtheta in a time-dependent manner.
None
The antibody is very good and it gives a single non-specific band.
Need high concentration
The antibody is specific and not much background.