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PE anti-human CD73 (Ecto-5'-nucleotidase) Antibody
344004
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The aim of this study was to identify ectonucleases on T cells in human peripheral blood. We chose this antibody because of the promised clear staining and because of the price.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this, peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and then resuspended in 300ul of FACS buffer and used for flow cytometry.
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Flow cytometry
This antibody is an excellent tool to identify CD73+ T cells in human peripheral blood.
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Good staining of CD73+ T cells
The population of CD73+ T cells are clearly identified with this antibody.