Santa Cruz Biotechnology
GRK 2 Antibody (C-9)
sc-13143
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The goal of this experiment was to test the endogenous expression level of GRK2 kinase in NIH3T3 cells. GRK2 phosphorylates a wide variety of GPCRs, a process necessary for receptor desensitization. This antibody was chosen because it was widely cited in many published papers.
Western Blot
NIH3T3 cells
1:1000 in blocking agent for 16 hr at 4 deg
5% milk powder in 1x TBS-T (0.1% Tween)
Donkey anti-mouse IgG HRP used at 1:5000 dilution in blocking buffer for 1 hr at room temp
NA
ECL 15 sec
Whole cell extract of NIH3T3 cells was prepared in RIPA lysis buffer and 50 µg of protein was loaded for SDS-PAGE followed by Western Blotting with GRK2 antibody from Santa Cruz Biotechnology. This antibody detects a band at ~75 kDa (the expected size for GRK2).
None
Specific signal was detected
This is a wonderful antibody for detection of GRK2 in Western Blotting