Immunocytochemistry of DRP1

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-DRP1

Catalog Number:

8570S

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We were testing this antibody to localize DRP1 in cancer cells through immunocytocytochemistry.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cancer cells

Primary Incubation

1:200 in 2.5% goat serum, overnight at 4 C

Blocking Agent

2.5% goat serum

Secondary Incubation

1:300, one hour at room temperature

Tertiary Incubation

NA

Detection

Olympus confocal microscoppy

Results Summary

The antibody worked very well for ICC staining of DRP1 in cancer cells.

Additional Notes

None

Related Categories

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Summary

The Good

Very good antibody for ICC and western blotting as well.

The Bad

None

The Bottom Line

Good antibody for detecting DRP1 through both western blotting and ICC.

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