NR5A2 Staining in Human Pancreas

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

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Company:

Sigma-Aldrich, powered by Atlas Antibodies

Product Name:

Anti-NR5A2 antibody produced in rabbit

Catalog Number:

HPA005455

The Nuclear Receptor subfamily 5, group A, member 2 (NR5A2) is a member of the family of nuclear receptors that function as regulatory transcription factors. NR5A2 is expressed in pancreas, liver, intestine, ovary and breast adipose tissue. It plays a role in embryonic development, cell differentiation, bile acid metabolism and steroidogenesis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

19 Year old human pancreas; Cryosection

Primary Incubation

1:1000, 4 Deg C, overnight.

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS (w/v)

Secondary Incubation

1:1000. rabbit IgG-Cy2. 2 hours, Room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

NR5A2 antibody stained well at 1:1000. It appears ubiquitous throughout the pancreas, staining nuceli of both endocrine (Insulin, Glucagon) and exocrine cells.

Additional Notes

None

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Summary

The Good

Works well at 1:1000 with little background.

The Bad

None

The Bottom Line

This antibody from Sigma works well at 1:1000 with little background.

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