Dynein Cytoplasmic Antibody in Immunofluorescence

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

AbD Serotec

Product Name:

Mouse Anti Bovine Dynein Cytoplasmic 74kDa

Catalog Number:

0100-0076

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Caco-2 cells

Primary Incubation

1:100 diluted in 1xPBS, incubation for 1h at room temperature

Blocking Agent

No blocking

Secondary Incubation

Cy3-coupled secondary antibody, 30 min incubation at room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

We were able to detect the cytoplasmic staining for the cytoplasmic dynein complex in the cells.

Additional Notes

None

Image Gallery

Summary

The Good

Very nice staining

The Bad

Nothing

The Bottom Line

Great antibody for the detection of cytoplasmic dynein.

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