Abcam
Anti-KAT4/TBP Associated Factor 1
ab75656
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The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.
Immunofluorescence
Caco-2 cells
1:200 diluted in 1xPBS, incubation for 1h at room temperature
No blocking
Cy3-coupled secondary antibody, 30 min incubation at room temperature
NA
Fluorescence microscopy
We were able to detect the nuclear localization of the specific KAT4 protein in Caco-2 cells with immunofluorescence.
None
Specific nuclear staining
Nothing
Great antibody to detect the nuclear localization of KAT4 with immunofluorescence.