Goat Anti-Mouse IgG-HRP for Western Blot

September 04, 2015

Project JRF
Centre for Cellular & Molecular Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Goat anti-mouse IgG-HRP

Catalog Number:

sc-2005

Info: View Product View Product Specs

Browse Similar Products: IgG-HRP Antibodies

Our aim was to check the expression of cancer stem cells and differentiation markers in glioblastoma cells. This product is highly specific with high dilution ratio and less expensive than others relatively.

Experimental Design and Results Summary

Applications

Western Blot

Sample

U87

Primary Incubation

Beta-actin, 1:5000 in 1xTBST, 4 degrees centigrade, Overnight incubation.

Blocking Agent

BSA in 1xTBST, Room temperature, 1h.

Secondary Incubation

1:10000, Room temperature, 1h.

Tertiary Incubation

No Tertiary incubation

Detection

Chem-doc

Results Summary

The goat anti-mouse IgG-HRP with 1h incubation at Room temperature gives good signal in chem-doc.

Additional Notes

None

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Summary

The Good

Less expensive, high dilution ratio, more specific.

The Bad

No drawback with the product.

The Bottom Line

Highly recommended to use.

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