Review for Anti-alpha Smooth Muscle Actin Antibody

February 01, 2016

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-alpha smooth muscle actin antibody

Catalog Number:

ab5694

We injected adipose-derived stem cells into mice to detect the wound healing effects and signaling cascade involved. Several markers are chosen including alpha smooth muscle, Cytokeratin 10, Ki67, and Wnt11. We chose Abcam antibodies since the company has a good reputation of good quality.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293

Primary Incubation

4 degrees celcius overnight

Blocking Agent

5% milk

Secondary Incubation

Goat anti-rabbit secondary antibody

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

Clear, specific band observed

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

NA

The Bottom Line

Works as expected

Share your experience with other scientists. Write a Review! »

Join the discussion