Anti-Galpha 11 Antibody for Western blot and Immunoprecipitation

June 30, 2015

Physiology
University of Tennessee Health Science Center

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-Galpha 11 antibody

Catalog Number:

sc-390382

Info: View Product View Product Specs

Browse Similar Products: Galpha 11 Antibodies

This antibody was used to study the role of chemokines on GPCR receptor activation. I used this antibody for an Immunoprecipitation (IP) blot. It is good as I don't see any IgG bands and it gives a single sharp band without any non-specific background. I have selected this antibody because it is mouse monoclonal & my IP was with rabbit polyclonal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HASMC lysates_IP with CCR4 antibody (Rabbit polyclonal)

Primary Incubation

Antibody at 1:1000 dilution; at 4 degrees overnight in 5% skim milk powder in 0.1% tween-TBS

Blocking Agent

At RT for 2 hrs in 5% skim milk powder in 0.1% tween-TBS

Secondary Incubation

At RT for 1 hr in 5% skim milk powder in 0.1% tween-TBS & 1:5000 anti-mouse-conjugated with HRP;

Tertiary Incubation

NA

Detection

Chemiluminescence

Results Summary

To check the association of CCR4 with Galpha 11 protein. There is no association/dissociation between CCR4/Ga11

Additional Notes

None

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Summary

The Good

Very specific. Doesn't detect any IgG in IP blot

The Bad

I don't see any drawback but primary dilution is little high

The Bottom Line

The antibody works very good and gives a single specific band. It doesn't detect IgG bands which is good for those interested in doing IP experiments.

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