Use of MACS separation columns in cell culture

May 01, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

Overall

Performance

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

MACS Separation Columns

Catalog Number:

130-042-401, 130-042-901, 130-042-201

I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. MACS separation columns are used to separate CD4+ T cells from CD8+ T cells.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Naive T cells

Protocol Overview

Naive T cells are incubated with antibody. The T cell/antibody cocktail is then run through the MACS Separation columns where the CD8+ T cells will stick to the columns and the CD4+ T cells will be eluted.

Tips

Do not put too many cells in one column.

Results Summary

The columns work fairly well at separating the CD4+ from the CD8+ cells, but the cell yield is very low from these columns.

Features Summary

None

Additional Notes

None

Image Gallery

Summary

The Good

The column is extremely easy to use and set up.

The Bad

The cell yield is not very high from these columns.

The Bottom Line

These columns are a good option if you are starting out with a plethora of cells as you will lose most of them. If not, I would not recommend the use of these columns.

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