Fitzgerald
Rabbit polyclonal NPTII antibody
20C-CR1112R
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We used the antibody to determine amounts of neomycin phosphotransferase type II (NPTII) from E. coli in samples of transgenic Arabidopsis thaliana plants. NPTII confers resistance against the antibiotic kanamycine and this antibody is one of the very few functional, commercially available ones to be used as anti-GMO reagent. We analyze samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. Antibody works with high specificity on multiple samples containing crude extract of transgenic Arabidopsis thaliana. The picture shows the following lines expressing E. coli NPTII to test antibody specificity: ku70 (Riha et al. (2002)), prt6-2 (SALK_051088, aka prt6-5 in Graciet et al. (2009), and prt6-1 in Holman et al. (2009), and mpk1 Bartels et al. (2009)). A plant sensitive for kanamycin was used as a negative control.
Western Blot
Arabidopsis seedlings or leaves
1:1000
5% milk TBST
1:2500
NA
ECL
Very clear, specific, and distinct bands of the correct size, see comments in abstract.
None
Excellent antibody, specific and clear, one of the few available ones that works!