BioLegend anti-MHCII FITC antibody

DBS
University of Montana
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse I-A/I-E (MHC Class II) FITC Antibody

Catalog Number:

107606

Info: View Product View Product Specs

Browse Similar Products: I-A/I-E Antibodies

We used this product to asses effects of Aryl hydrocarbon receptor activation in dendritic cells and T cells. We assessed AhR activation in whole spleen primary cultures. We used FITC MHCII as part of our staining panel for dendritic cells, and potentially macrophages.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole splenocytes

Primary Incubation

25 minutes on ice, protected from light

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSAria II

Results Summary

We consistently get clean results with this antibody, and almost solely use it for MHCII detection.

Additional Notes

None

Related Categories

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Summary

The Good

Clean and consistent results

The Bad

None

The Bottom Line

I recommend this antibody. We almost solely use it for MHCII detection, because we are confident in its binding and specificity.

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