eBioscience
Foxp3 staining kit
00-5523-00
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This product was used while running experiments looking at pathogenic T cells in a lung infection model. We were trying to distinguish Th17 and Treg cells in lung tissue. Using this product, we have successfully stained foxp3, rorgt and other difficult transcription factors. This buffer works even better if you stain overnight with your intracellular stains. I've also found that you can cut the buffer and perm wash down by half volume and it still works great! I chose this product because it was far superior to the competitor's buffer set in a side by side comparison.
Flow cytometry
Lung tissue
Homogenized lung, stain 4x10^6 cells for surface markers, transcription factors and cytokines. Assess by flow cytometry.
Leave in perm buffer with intracellular antibodies overnight
Was able to identify IL-17+RORgt+ CD4+ cells from whole Lung homogenate following infection. Also staining of foxp3 on eF450 (pacific blue) was greatly improved over other buffer systems.
None
Staining works great for foxp3
Longer incubation times for best staining
Buy it! You won't regret it!
Product Name: Foxp3 / Transcription Factor Staining Buffer Set KitSupplier Page