SANTA CRUZ BIOTECHNOLOGY, INC
AlaRS (E9)
sc-165991
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We wanted to detect alanyl tRNA synthetase in different human cell lines and AlaRS (E9) was our choice of antibody from Santa Cruz. The polyclonal antibody worked really well at 1:2000 dilution (incubated overnight) and it was very specific. Though at higher exposure we saw some lower molecular weight bands appearing but that happens with any antibody. I have also used the antibody for immunofluorescence at 1:200 dilution and it worked well.
Western Blot
HEK and U937 cell lysate
1:2000 dilution with overnight incubation in cold room.
5% non fat dry milk solution
1:10000 (GE Healthcare) dilution with two hours incubation at room temperature.
None
ECL prime reagent from GE Healthcare
We got to see specific 107 kDa band after 15 seconds of exposure of the X-ray film. Longer exposures (>1 minute) resulted in appearance of non-specific bands of lower molecular weight.
The company asked us to use 1:1000 dilution but 1:2000 worked well for us.
Very strong antibody
Lower molecular weight non-specific bands.
Good antibody if your sole purpose is to detect the protein from cell lysate.