HNRNPC Antibody in A549 Using Western Blot

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Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

Company:

Aviva Systems Biology

Product Name:

HNRNPC antibody – middle region (ARP40517_P050)

Catalog Number:

ARP40517_P050

HNRNPC - Western Blot detection in A549 (human Aveolar Basal Epithelial Cells)

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lysate of A549 cells

Primary Incubation

Over night, 4°C , (1:500 – 1:1000)

Blocking Agent

5 % low-fat milk solution

Secondary Incubation

1h, room temperature, goat- anti-rabbit – HRP conjugated (DAKO, 1:5000)

Tertiary Incubation

None

Detection

ECL, x-ray films

Results Summary

One band at 34 kDa

Additional Notes

None

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Summary

The Good

Pricing, quality

The Bad

Nothing

The Bottom Line

Adequate tool

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