Aviva Systems Biology
FXYD5 antibody - N-terminal region
ARP37690
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The antibody reacts only with the human protein (not cross-reaction with the mouse protein); there is a very big not specific band around 10kDa that can be confusing, the specific band is around 30kDa.
Western Blot
293T overexpressing hFXYD5 or mFxyd5
1:1000 ON 4°C
5% milk
Rabbit-HRP 1:4000 1h RT
None
Homemade ECL
The antibody reacts only with the human protein (not the mouse protein, see no signal in lane 4); there is a big not specific band around 10kDa but I suppose the specific one is the one around 30kDa (indicated by arrow, positive control lane 5)
1.human Hela cells (50ug); 2.humanSKNBE cells (50ug); 3.human H1299 cells (50ug); 4.human293T transfected with mouse FXYD5 (10ug); 5.human 293T transfected with human FXYD5 (10ug); 6. human 293T (10ug)
Signal is clean arounf the specific 30kDa band
Unspecific band at 10 kDa
Good, not tested regarding the detection of the endogenous protein