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This antibody was used in an experiment to determine which on the two tested antibodies works best with primary human keratinocytes.
Primary human keratinocytes
Dilute 1:20, at room temperature, in the dark, for 30 min.
5% goat serum, 5% human AB serum in 1x BD permeabilization reagent
N/A
Facscalibur; CellQuest Pro software version 5.2.1
This antibody worked very well with the cells. There was hardly any background compared to the isotype control.
None
Great for facs analysis. A secondary antibody is not needed.
This antibody has only been tested for Facs analysis.
This antibody works well in labeling Krt 14-expressing cells for facs analysis.