Pfu Polymerase is a thermostable DNA polymerase isolated from the archaeal thermophile Pyrococcus furiosus. The 90-kDa Pfu polymerase enzyme features a 3’-5’ exonuclease proofreading ability, allowing for higher fidelity than Taq. The error rate of Pfu has been reported to be at least 10-fold lower than that of Taq. However, Pfu amplifies at a slower rate than Taq. Thus Pfu polymerase is suitable for DNA amplification applications requiring greater accuracy.