Laboratories operating under GMP or GLP regulations must follow guidelines set by regulatory agencies to protect scientific integrity or demonstrate quality assurance of manufactured products.
GMP and GLP labs using microplate readers in their workflows need to verify that the readers are functioning within their manufacturing specifications. Our SpectraTest ABS2 Validation Plate fulfills this requirement by helping you validate the optical performance of your reader in an automated, comprehensive, and traceable manner.
Eight different automated tests are provided - Absorbance accuracy; Precision; Stray light; Wavelength accuracy; Wavelength repeatability; Ultimate dark; Optical alignment; and Baseline noise.
Our readers are tested according to the highest quality standards to guarantee that they fulfill the specifications when they leave our factory. Local quality and GLP regulations require proven evidence that all lab equipment is working as expected.
To fulfill this requirement for our readers, we have developed our NIST traceable validation plate. Each filter is individually tested across all eight channels of the plate. Calibration of the plate’s filter standards is accomplished through the use of an instrument calibrated with primary NIST standards.
They are easy to use and help ensure that your reader is working consistently at high performance. The ABS2 plate will help you validate your reader conducting a range of different tests.
In column 1 and 2 the optical alignment and dynamic range of the light beam of your reader are tested. This test makes sure that the light beam goes through your sample and does not touch any well walls. To test the photometric accuracy, precision and linearity, columns 3-6 contain filters with increasing optical density.
Multiple measurements are conducted at four different optical densities. Afterwards, the mean variation and the linearity is calculated. Columns 7 -10 contain filters to test stray light. This test is to make sure that only the light of the correct wavelength is leaving the monochromators.
Finally, wavelength accuracy and precision are measured at position 11 & 12. Our microplate readers are designed to provide consistent performance for many years. However, in keeping with best practices, microplate reader performance should be validated and documented regularly.