Protein Standards and Ladders

Protein Standards and Ladders Our catalog lists protein ladders, standards, and markers from several suppliers for measuring protein molecular weights via electrophoresis. Methods for separating and detecting proteins, such as SDS-PAGE, native PAGE, 2-D electrophoresis, and Western blotting, require the loading of a ladder to determine the relative sizes of proteins. Commercial size markers are generally premixed and ready-to-use, and can come in a variety of options. Prestained protein ladders allow the user to continuously monitor the progression of electrophoresis. It can also let the user quickly assess the efficiency of blotting. Broad range markers contains proteins with low to high molecular weights, usually ranging between 10 to 240 kilodaltons. If the proteins of interest are known to be large or small, consider using high or low range markers, respectively.