NGS has fundamentally changed genomics research. It enables scientists to sequence an entire genome, transcriptome, or exome, conducting experiments that were previously impossible. However, a number of challenges remain with this technology. The short-read length that this technology relies on is not well-suited for de novo assembly, as the data isn’t easily reconstructed without a reference genome. Deep sequencing partially compensates for this limitation by minimizing errors, but repetitive regions are still a challenge. Sample preparation for NGS can also be labor-intensive. In this guide, learn about the future of NGS technology, solutions to the challenges posed by NGS, and find protocols and tips to take you from sample prep to sequencing and analysis.

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NGS Guide: From Sample Prep to Analysis

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