Full Automation of a Covaris-Integrated High Throughput Cell-Based Assay System
Saeed Seyedmohammad, Ph.D.
Thursday, March 9, 2023
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Chapters
Introduction
Full Automation of a Covaris-Integrated High Throughput Cell-Based Assay System
Background Research Context
Full Automation of a Covaris-Integrated High Throughput Cell-Based Assay System
Establishing the Human Cardiomyocyte Cell Line for HTP Cell-Based Assay Protein Extraction
Scaling Cardiomyocyte Culture to a Functional HTP Assay
Integrating Automation Following Cell Culture – Covaris LE220 Plus System
Integrating Automation Following Cell Culture – Manual Verses Automation
Integrating Automation Following Cell Culture – Proteomic Dynamic Range of AC16 Cells Grown from 96-Well Plate
4 key stages workflow
Full Automation of a Covaris-Integrated High Throughput Cell-Based Assay System
Assessing Cellular Proteome Coverage in a Perturb System using our Fully Automated HTP Cell System – I/R Cardiac Injury
Assessing Cellular Proteome Coverage in a Perturb System using a Fully Automated HTP Cell System – I/R Injury
Full Automation of a Covaris-Integrated High Throughput Cell-Based Assay System
Summary Points
Future Directions
Thank You
Jenny Van Eyk Lab
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