Watch Webinar: Characterizing Functions of Immune Cells Using Live-Cell Analysis
Thursday, May 17, 2018
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Chapters
Lindy O’Clair Introduction
Live-cell Imaging and Analysis using the IncuCyte® S3
IncuCyte® S3 Live Cell Analysis
Real-time Analysis of Immune Cells
Characterizing Functions of Immune Cells using Live-cell Analysis
Gillian Lovell, Ph.D. Introduction
Innate immune response
NETosis
Live-cell analysis of NET release
Workflow
Protocol
Validate protocol using cell line model
Cellular mechanisms: ROS burst, nuclear decondensation, NET release
Use of primary cells
Pharmacological inhibition of NETosis
Live-cell analysis of Phagocytosis
Live-cell analysis of neutrophil function
Phagocytosis
Conclusion
Chia-Wei Li Ph.D. Introduction
Subtype of breast cancer
PD-L1 expressed at 50 kDa
The 50 kDa PD-L1 is abundant in TNBC
Tunicamycin reduces PD-L1 protein migration
Protein glycosylation
Glycosylation of membrane glycoprotein
PD-L1 is glycosylated at consensus NXT motif
Glycosylation of PD-L1 stabilizes PD-L1 protein
PD-L1 contains a GSK3β phosphorylation degron
Glycosylation of PD-L1 suppresses T cell activity
Can gPD-L1 be a therapeutic target?
Glycosylation is required for PD-L1-PD-1 interaction
STM108 internalizes PD-L1
Targeting PD-L1 glycosylation enhances anti-tumor immunity
Anti-gPD-L1 ADC eradicates TNBC
Conclusion
Glycosylation is required for co-inhibitory receptor interaction
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