Live Cell Assay Approaches for Immunology and Immuno-oncology: Cell Health, Chemotaxis Immune Cell Killing, and More…
Daniel M. Appledorn, PhD
Tuesday, May 23, 2017
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
IncuCyte is a Revolutionary Cell Analysis Platform
IncuCyte® S3 – Live-Cell Imaging and Analysis around-the-clock
Automated Data Acquisition and Real-Time Analysis
The Power of Live-Cell Analysis
Unique Multi-Channel Image Acquisition
Remote Viewing, Control and Connectivity
Immune Cell Killing Application
Immune Cell Killing (cont’d) - Productivity
96-well Microplate view
ADCC in nonadherent target cells
Bispecific T cell molecules and donor variability
Immune Cell Killing – A Look Toward the Future
IncuCyte S3 – Optimized for 3D Spheroid Analysis
10x Spheroid Growth/Shrinkage (0 – 10 days)
Real-Time Analysis and Graphing
Immune Cell Proliferation & Clustering
Immune Cell Proliferation Coating Optimization
Coatings provide a semi-adherent surface aiding retention of cells in the field of view
IncuCyte can quantify proliferation of non-adherent cells
Probing the mechanisms of T cell activation
Chemotaxis and Transendothelial Migration
Video Clip - Essen Chemotaxis Plate
IncuCyte Chemotaxis Chamber
Video Clip - Adherent Cells
Kinetic inhibition and pharmacology of AMD3100
Transendothelial Migration – Immune cells migrating through a HUVEC monolayer
Video Clip - HUVEC monolayer
LFA-1/ICAM-1 Interaction is Required for Maximal TEM
Cell clearance - antibody dependent cell phagocytosis
Anti-CD47 induced phagocytosis of T-lymphoblasts
Labeled target cells fluoresce as they are internalized into BMDMs
Kinetics of ADCP is cell type dependent
Kinetics of phagocytosis is dependent on differentiation
IncuCyte is a Revolutionary Cell Analysis Platform
IncuCyte Key Applications
Key Resources
No transcript for this webinar
More Information
Contact Us