Watch Webinar: Using Shotgun Metagenomics to Reveal the Impact of the Gut Microbiome
Rob Knight, PhD
Wednesday, April 5, 2017
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
Human Genome
What if your gut came equipped with…
Microbiome science is revolutionizing how we see ourselves
What do you see when you look in the mirror?
43% human cells
1 teaspoon stool = 1 ton of DVDs
NIH Human Microbiome Project
How rapidly results moved from scientific journals to general publications
ATGC…
What can a doctor do with sequencing data?
Quantitative Insights into Microbial Ecology
– Interpretable map of microbial communities
American Gut Project
Embriette Hyde accepting “People’s Choice” award
Diversity increases with age, sleep
What do you think affects your gut microbiome?
Expanding the American Gut Project
Oral, skin, vaginal, fecal microbiome
Patients | Donor
Fecal transplant
Fecal transplant results
Microbiome transformation
Smart toilet to predict microbiome
Can I predict my health from my microbiome?
IBD Patient and Microbiome
Larry’s stool to Rob’s lab
Larry Smarr Weekly Weight
LS Gut Microbiome 16S Evolution by Family — No Clear Separation at January 2014
Time Development Over 3.5 Years of Larry Smarr’s Gut Microbiome Ecology
Larry Smarr Gut Microbiome Ecology Shifted After Drug Therapy
Pharmacometabonomic identification
Problems with 16S rRNA profiling
Why not move to shotgun?
Technology Platform – Increase throughput, decrease cost
How to measure microbiome functions?
SHOGUN: near-WGS species profiles
SHOGUN predicts genes not observed with deep shotgun
Miniaturizing workflow 10x using Echo
Shotgun Analysis of DNA Controls
Deplete Host DNA: Slow spin + PMA
Most important: Never have to explain OTUs to a clinician again!
Where are we using this?
The Future
Roll this into the previous chapter
Center for Microbiome Innovation
Acknowledgments
Members of Knight Lab
Questions?
No transcript for this webinar
More Information
Request info