Ultra-sensitive Sequencing for Liquid Biopsy Samples
Dr. Cristian Ionescu-Zanetti
Monday, December 5, 2016
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Chapters
Introduction
Application of liquid biopsy
Using Liquid Biopsy Samples to Detect Cancer Burden
The Signal-to-Noise Challenge: Approaches to Detecting Alterations Below 1%
cfDNA Experimental Design
Coverage Uniformity Across Sample Types
Spotlight 59 Panel
The Spotlight 59 Workflow
ERASE-Seq
Recurrent Artifacts
Stochastic Errors
Low AF calls – Traditional Workflow
Single sample calls: 0.5 – 1.5%
ERASE-Seq Caller Strategy
The ERASE-Seq confidence score
ERASE-Seq identifies true positives
Analytical tests: multiple tumor cell lines spiked into normal DNA
Improved sensitivity/specificity at the same overall depth (standard caller)
Spotlight 59 Performance as compared to published methods
The IsoFlux liquid biopsy workflow
Clinical Research: IsoFlux enriched CTC liquid biopsy
What’s Spotlight 59
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