Chromium™ Single Cell Solutions
Dr. Tarjei Mikkelsen
Tuesday, November 29, 2016
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Chapters
Introduction
Fully integrated solutions for DNA and Single Cell analysis
Single Cell Analysis: Biology in HD
Single Cell Partitioning in GEMs
Single Cell Partitioning, Lysis and Barcoding
Single Cell 3’ Digital Gene Expression
Single Cell 3’ Application Highlights pt.1
Single Cell 3’ Application Highlights pt.2
Chromium™ Single Cell 3’ Solution v2
Refined Microfluidics
New Gel Bead RT Primers for Inline Barcoding
Shortened and Optimized Workflow
Improved Sensitivity: More Distinct Genes per Cell
Improved Sensitivity: More Transcripts per Cell
Compatibility with All Illumina Sequencers
Cell Ranger™ 1.2
Million-scale Single Cell Analysis
Million-scale Single Cell Analysis in Perspective
1,308,421 Cells pt.1
1,308,421 Cells pt.2
1,308,421 Cells pt.3
Data Release
Chromium™ Single Cell Controller
Single Cell, Full-length V(D)J Sequencing
Single Cell V(D)J Sequencing: Immune Systems in HD pt.1
Single Cell V(D)J Sequencing: Immune Systems in HD pt.2
New Solution from Proven Components
Observed Diversity in Clonal vs Non-Clonal Cell Populations
Paired TCR Alpha and Beta V(D)J Sequences (Jurkat)
Paired TCR Alpha and Beta V(D)J Sequences (Primary T Cells)
Summary
THANK YOU! Team 10x
10x Genomics
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