Streamline your RNA-seq and Target Enrichment NGS library preparation with KAPA HyperPrep
Nancy Nabilsi, PhD
Wednesday, December 7, 2016
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Chapters
Introduction
OUR GOAL
Combining Best-in-Class Technologies for End-to-End Solutions
KAPA Library Preparation Kits and Enzymes
Addressing a Sample Preparation Wish List
Diagram
Fundamentals of DNA-seq Library Construction
Streamlining DNA Library Prep
Generate libraries that retain the complexity of the original sample—in less time
KAPA Library Preparation Kits and Enzymes
In a recent publication comparing 9 commercially available kits
NimbleGen Target Enrichment Products
What is HyperCap?
Introducing HyperCap
HyperCap Workflow Improvements
HyperCap Performance — Cancer Panel
HyperCap Performance — MedExome
HyperCap
Multiple automated solutions are supported and being developed with our collaborators
HyperCap Performance — Cancer Panel
Optional: HyperCap “Extreme”
HyperCap Extreme Performance — MedExome
The Evolution of Target Enrichment workflows
The Evolution of KAPA Library Prep for RNA-seq
RNA-seq Workflow Streamlining
RNA Enrichment Strategies
Performance: High-quality, Low-input
Sequencing metrics
RNA Enrichment Strategies
Impact of Sample Quality on Library Construction
Performance: Reproducibility
Performance: Efficiency and Accuracy
Performance: Sequencing Metrics
Performance: Differential Gene Expression
KAPA RNA HyperPrep
Summary
Acknowledgements
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