Fig 1: Downregulation of miR-138-5p or SIRT1 knockdown alter cell viability, apoptosis and the expression of inflammatory factors. PC12 cells were transfected with inhibitor control, miR-138-5p inhibitor or miR-138-5p inhibitor + SIRT1-siRNA for 48 h; then, these cells were subjected to LPS treatment (100 ng/ml) for 4 h. (A) Cell viability was measured by MTT assays. (B) Histogram of the percentage of early and late apoptotic cells. (C) Flow cytometric analysis was conducted to evaluate cell apoptosis using Annexin V/PI double staining. Inflammatory factors including (D) TNF-a, (E) IL-1ß and (F) IL-6 were detected via ELISA. Data are presented as the mean ± SD. The experiments were performed in triplicate. **P<0.01 vs. Control; ##P<0.01 vs. LPS; &&P<0.01 vs. LPS + inhibitor. miR-138-5p, microRNA-138-5p; SIRT1, sirtuin 1; siRNA, small interfering RNA; LPS, lipopolysaccharide; IL, interleukin; TNF, tumor necrosis factor; PI, propidium iodide.
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