Fig 1: Identification and ELISA Verification of CHI3L1 as a Highly Elevated Secreted Protein in GC Plasma(A) A Venn diagram showed that 58 out of 94 database-derived upregulated genes were secretome or membrane associated. (B) There was only 1 gene, CHI3L1, characterized as upregulated in both the database-derived list and SWATH-MS. (C) An overall Venn diagram showed the overlap among GC dataset consensus DEGs, SWATH-MS DIA-identified DEGs in GC plasma, membrane protein lists, and secretome. (D) A boxplot showed different CHI3L1 levels in plasma of patients with GC, patients with BN, patients with HPs, and NCs by ELISA. (E) ROC plots exhibited the efficiencies of CHI3L1 in distinguishing GC patients from those with precancerous BN, those with HPs, NCs, and all of the above.
Fig 2: Integrating CHI3L1 with Clinical Factors Could Well Distinguish Patients with Early-Stage Endoscopically Resectable GC from Others(A) The ROC plot of stage-I GC patients versus all other participants. (B) The ROC plot of stage-I GC patients versus BN patients. (C) The ROC plot of stage-I GC patients versus those with HPs. (D) The ROC plot of stage-I GC patients versus NCs.
Supplier Page from CUSABIO Technology LLC for Human Chitinase-3-like Protein 1(YKL-40/CHI3L1)ELISA Kit