Zombie Green(TM) Fixable Viability Kit 423111 from BioLegend

Product Specs
ItemZombie Green(TM) Fixable Viability Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423111
Quantity100 tests
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epitheliaiScienceAugust 18, 2023Diana Cadena Castaneda, Sonia Jangra, Marina Yurieva, Jan Martinek, Megan Callender, Matthew Coxe, Angela Choi, Juan García-Bernalt Diego, Jianan Lin, Te-Chia Wu, et al.Aqua™ Fixable Viability Kit,Biolegend,Cat#423101 Zombie Green™ Fixable Viability Kit,Biolegend,Cat#423111 Saponin,Sigma,Cat#S7900-100G ,, Critical commercial assays,Critical commercial assays,Critical commercial

(2) TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisationJournal of translational medicineAugust 14, 2023Wu Y, Zhan S, Chen L, Sun M, Li M, Mou X, Zhang Z, Xu L, Xu Y.tube) were primarily labelled with by the Zombie Green™ Fixable Viability Kit (BioLegend, CA, USA, cat#423111) for 15 min protected from light. After washing with staining buffer (BioLegend, CA, USA, cat#420201),

(3) Proteomics of immune cells from liver tumors reveals immunotherapy targetsCell GenomicsJune 14, 2023Fernando P. Canale, Julia Neumann, Janusz von Renesse, Elisabetta Loggi, Matteo Pecoraro, Ian Vogel, Giada Zoppi, Gaia Antonini, Tobias Wolf, Wenjie Jin, et al.human interleukin-2,Sino Biological,Cat#11848-HNAH1-E Zombie Green Fixable Viability Kit,Biolegend,Cat#423111 Zombie UV Fixable Viability Kit,Biolegend,Cat#423107 CellTrace™ Violet Cell Proliferation Kit,ThermoFisher,Cat#C34557 CellTrace™

(4) Infection with hypervirulent Mycobacterium tuberculosis triggers emergency myelopoiesis but not trained immunityFrontiers in ImmunologyJune 13, 2023Ana Raquel Maceiras, Diogo Silvério, Rute Gonçalves, Marcos S. Cardoso, Margarida Saraivacells were excluded from the analyses using ZombieAqua (Cat. #423102, Biolegend) or ZombieGreen (Cat. #423112, Biolegend) viability dyes. Cells were acquired on a BD FACS Canto II or BD Fortessa II. Data were analyzed

(5) Increased Bone Marrow Uptake and Accumulation of Very-Late Antigen-4 Targeted Lipid NanoparticlesPharmaceuticsMay 27, 2023Laura E. Swart, Marcel H. A. M. Fens, Anita van Oort, Piotr Waranecki, L. Daniel Mata Casimiro, David Tuk, Martijn Hendriksen, Luca van den Brink, Elizabeth Schweighart, Cor Seinen, et al.152203), 1:200 mCD49d-APC (clone R1-2, cat# 103621) and 1:100 Zombie GreenTM Fixable Viability Kit (cat# 423111) in 50 µL Trustain FcX 1:250 in PBS; all antibodies were obtained from BioLegend. After incubation for

(6) LMAN1 is a receptor for house dust mite allergensCell reportsMarch 28, 2023Miller MH, Swaby LG, Vailoces VS, LaFratta M, Zhang Y, Zhu X, Hitchcock DJ, Jewett TJ, Zhang B, Tigno-Aranjuez JT.ab269823 α-FLAG affinity resin,Thermo Scientific,Cat# A36803 Zombie green viability dye,Biolegend,Cat# 423112

(7) Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda SignalingVirusesFebruary 25, 2023Evans JG, Novotny LA, Meissner EG.(forward and side scatter) and by staining with a viability dye (1:1000, Zombie Green Fixable Viability, 423111 or 1:1000, Zombie Red Fixable Viability, 423109, BioLegend). FlowJo software was used for analysis. 2.4.

(8) High cell density and high-resolution 3D bioprinting for fabricating vascularized tissuesScience AdvancesFebruary 24, 2023Shangting You, Yi Xiang, Henry H. Hwang, David B. Berry, Wisarut Kiratitanaporn, Jiaao Guan, Emmie Yao, Min Tang, Zheng Zhong, Xinyue Ma, et al.(Leica DMI6000B). Cell viability in the thick tissue was characterized by a Zombie Green viability kit (423111, BioLegend). The tissue was cultured in a perfusion culture system for 14 days and then harvested. A

(9) Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and FreezingVeterinary SciencesFebruary 21, 2023Giorgia Podico, Kianna M. Spencer, Humberto B. Magalhaes, Igor F. Canissoinvestigators of the present study [9,19,20,21]. Briefly, the staining solution of Zombie Green dye (#423112 BioLegend, San Diego, CA, USA) was freshly prepared with 100 μL of DMSO added to each vial of dye. Similarly,

(10) SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of miceiScienceFebruary 17, 2023Gonçalves R, Couto J, Ferreirinha P, Costa JM, Silvério D, Silva ML, Fernandes AI, Madureira P, Alves NL, Lamas S, et al.CD8a,Biolegend,"Cat#100741 (53-6.7, 1:200); RRID:AB_11124344" Zombie Green™ Fixable Viability Kit,Biolegend,"Cat#423112 (-, 1:500); RRID:AB_2927787" FITC anti-mouse CD8a,Biolegend,Cat#100804 (5H10-1; 1:200); RRID:AB_312765 "TCR gamma/delta

(11) Endothelial Toll-like receptor 4 is required for microglia activation in the murine retina after systemic lipopolysaccharide exposureJournal of neuroinflammationFebruary 4, 2023Tsioti I, Steiner BL, Escher P, Zinkernagel MS, Benz PM, Kokona D.cells stained with Zombie Green™ Fixable Viability Kit (1:800; Biolegend, San Diego, CA, USA; Cat # 423111) were excluded from the analysis. Extracellular markers against the leukocyte common antigen CD45 (30-F11;

(12) Single-cell RNA sequencing identifies a population of human liver-type ILC1sCell reportsJanuary 31, 2023Krämer B, Nalin AP, Ma F, Eickhoff S, Lutz P, Leonardelli S, Goeser F, Finnemann C, Hack G, Raabe J, et al.proteins" IL2(IS),Miltenyi,Cat#130-097-748 IGF-β1,Miltenyi,Cat#130-095-066 IL-7,Miltenyi,Cat#130-095-363 IL-15,Immunotools,Cat#11340155 IL-12,Immunotools,Cat#11349125 Viability Zombie Green,Biolegend,Cat#423112 viability Zombie-Aqua,Biolegend,Cat#423102 FLT3L,Immunotools,Cat#11343303 Medium F-12 Nut Mix (+)

(13) In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinomaNature communicationsJanuary 17, 2023Taniguchi S, Matsui T, Kimura K, Funaki S, Miyamoto Y, Uchida Y, Sudo T, Kikuta J, Hara T, Motooka D, et al.cell strainer and centrifuged at 300 × g for 5 min to prepare single cell suspensions. Zombie Green (#423111, BioLegend) was used to remove dead cells. Blood and isolated cells were blocked with anti-CD16/32 antibody

(14) Cancer-targeted photoimmunotherapy induces antitumor immunity and can be augmented by anti-PD-1 therapy for durable anticancer responses in an immunologically active murine tumor modelCancer Immunology, ImmunotherapyJanuary 1, 2023Michelle A. Hsu, Stephanie M. Okamura, C. Daniel De Magalhaes Filho, Daniele M. Bergeron, Ahiram Rodriguez, Melissa West, Deepak Yadav, Roger Heim, Jerry J. Fong, Miguel Garcia-Guzmancell dissociation buffer. Single-cell suspensions were stained with Zombie Green (Biolegend, Cat. No. 423111) for 10 to 15 min at room temperature in the dark. Cells were washed prior to the addition of surface

(15) Obesity reduced survival with 5-fluorouracil and did not protect against chemotherapy-induced cachexia or immune cell cytotoxicity in miceCancer Biology & TherapyDecember 31, 2022Brandon N. VanderVeen et al.was prepared in PBS and stained for dead cells using Zombie Green™ (BioLegend, San Diego, CA; Cat#: 423111) following the manufacturer’s instruction. Cells were then suspended in flow buffer (5% FBS, 100 mM

(16) Perfluorodecalin-based oxygenated emulsion as a topical treatment for chemical burn to the eyeNature communicationsNovember 30, 2022Li S, Pang K, Zhu S, Pate K, Yin J.used for HIF-1α staining. Live and dead cells were stained using Zombie Green Fixable viability kit (423111, BioLegend). The stained cells were analyzed using the LSRII flow cytometer (BD Biosciences, San Jose,

(17) Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapyCancer cellNovember 14, 2022Li K, Tandurella JA, Gai J, Zhu Q, Lim SJ, Thomas DL 2nd, Xia T, Mo G, Mitchell JT, Montagne J, et al.TILs and peripheral blood mononuclear cells were stained with Zombie Green viability dye (Biolegend, 423111), anti-CD4-PE-CF594 (Biolegend, 562281), anti-CD8-APC (Biolegend, 344722), anti-CD19-PE (Biolegend,

(18) Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenesNature communicationsOctober 14, 2022Mayer RL, Verbeke R, Asselman C, Aernout I, Gul A, Eggermont D, Boucher K, Thery F, Maia TM, Demol H, et al.(Li-COR). For flow cytometry analysis, cells were first stained with fixable viability dye Zombie green (#423111, Biolegend) and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding

(19) Immunization with a Prefusion SARS-CoV-2 Spike Protein Vaccine (RBMRNA-176) Protects against Viral Challenge in Mice and Nonhuman PrimatesVaccines (Basel)October 11, 2022Qinhai Ma, Runfeng Li, Jianmin Guo, Man Li, Lin Ma, Jun Dai, Yongxia Shi, Jinlong Dai, Yuankeng Huang, Cailing Dai, et al.stimulation, splenocytes were washed twice with PBS and the Zombie Green™ Fixable Viability Kits (BioLegend, 423111) was used to determine the viability of cells prior to the fixation and permeabilization. Cells were

(20) Self-reactivity of CD8 T-cell clones determines their differentiation status rather than their responsiveness in infectionsFrontiers in ImmunologyOctober 6, 2022Darina Paprckova, Veronika Niederlova, Alena Moudra, Ales Drobek, Michaela Pribikova, Sarka Janusova, Kilian Schober, Ales Neuwirth, Juraj Michalik, Martina Huranova, et al.Near-IR Dead Cell Stain Kit (Invitrogen #L34975), Zombie Green™ Fixable Viability Kit (Biolegend, #423111) or Hoechst 33258 (Invitrogen, #H3569). For the analysis of Jurkat cell lines, anti-CD8 (clone MEM-31,

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(1)

10/17/2023Superb Kit From BioLegend for Live/Dead Cell Analysis Danielle SchmidtDoes a great job staining dead cells for accurate live/dead analysis in flow cytometry. Be sure to keep it in the dark, as it is light-sensitive!
(1)

7/22/2023Great Viability Dye for Flow Cytometry From BioLegend Troy TrevinoInflammation in brain endothelial cells is measured during viral respiratory infection in mice. Zombie Green viability dye was used to exclude dead cells from analysis.
(1)

7/4/2019Very Good BioLegend Kit for Viability QC on FACS Evaristus MbanefoIt is a good product and serves the purpose. I will give 9/10.

Zombie Green(TM) Fixable Viability Kit from BioLegend

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Zombie Green(TM) Fixable Viability Kit from BioLegend

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