Description
The determination of chicken IgY is carried out as direct sandwich ELISA. An antibody specific for chicken IgY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgY present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgY con-cen-tration