Zombie NIR(TM) Fixable Viability Kit 423106 from BioLegend

Product Specs
ItemZombie NIR(TM) Fixable Viability Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423106
Quantity500 tests
ApplicationsFlow Cytometry (FC)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenesSTAR ProtocolsDecember 1, 2023Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen7-AAD Viability Staining Solution,BioLegend,Cat# 420404 Zombie NIR Fixable Viability Kit,BioLegend,Cat# 423105 Brain heart infusion (BHI),Oxoid,Cat# CM1135 Agar,Biosharp,Cat# BS195-1k Cell Activation Cocktail (with Brefeldin A

(2) Protocol for isolation of signet ring cells from human gastric mucosaSTAR ProtocolsDecember 1, 2023Sarah G. Samaranayake, Lauren A. Gamble, Cassidy Bowden, Benjamin L. Green, Amber F. Gallanis, Dilara Akbulut, Niharika Shah, Jonathan M. Hernandez, Jeremy L. Davisgastric mucosa demonstrates a viable sample. 80.1% viability with Zombie Live-Dead staining (BioLegend Cat#423105) with significant cytokeratin expression via Alexa Fluor 647-conjugated pan-cytokeratin monoclonal antibody

(3) Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulationFrontiers in ImmunologyNovember 20, 2023Zhao-Yuan Wang, Yu-Xin Zheng, Fan Xu, Ying-Zhe Cui, Xue-Yan Chen, Si-Qi Chen, Bing-Xi Yan, Yuan Zhou, Min Zheng, Xiao-Yong Man(1:100, BioLegend, San Diego, CA), the cells were incubated with Live/Dead Zombie dye (UV, BioLegend, 423106, 1:100) and antibodies specific for CD45 (BV510, BioLegend, 103138, 1:100) and FcϵRIα (PE/Cyanine7,

(4) PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancerCancer immunology, immunotherapy : CIINovember 1, 2023Kawasaki K, Noma K, Kato T, Ohara T, Tanabe S, Takeda Y, Matsumoto H, Nishimura S, Kunitomo T, Akai M, et al.were also used. Dead cells (1:1000 dilution) were stained using a Zombie NIR Fixable Viability Kit (423106, BioLegend). Stained cells were analyzed using flow cytometry (FACSLyric; BD Biosciences, Franklin Lakes,

(5) Radiotherapy combined with docetaxel alters the immune phenotype of HNSCC cells and results in increased surface expression of CD137 and release of HMGB1 of specifically HPV-positive tumor cellsNeoplasiaNovember 1, 2023Fridolin Grottker, Simon Gehre, Clara M. Reichardt, Azzaya Sengedorj, Tina Jost, Thorsten Rieckmann, Markus Hecht, Antoniu-Oreste Gostian, Benjamin Frey, Rainer Fietkau, et al.Diego, CA, USA" TNFRSF9/CD 137,BV 421,311508,BioLegend,"San Diego, CA, USA" Live/dead,Zombie NIR,423105,BioLegend,"San Diego, CA, USA" List of flow cytometry antibodies and dyes for immune checkpoint molecule

(6) CXCL13 promotes thermogenesis in mice via recruitment of M2 macrophage and inhibition of inflammation in brown adipose tissueFrontiers in ImmunologyOctober 23, 2023Lijun Xie, Huiying Wang, Dan Wu, Feng Zhang, Wei Chen, Yuqing Ye, Fang HuFlow cytometry Single cells of SVF fraction were resuspended with PBS and stained with zombie NIR™ dye (#423105, Biolegend, USA), blocked with FcX Blocking CD16/32 (#156604), and dyed with a mix of surface marker

(7) Tumor cell-induced platelet aggregation accelerates hematogenous metastasis of malignant melanoma by triggering macrophage recruitmentJournal of experimental & clinical cancer research : CROctober 23, 2023Chen Y, Zhou J, Liu Z, Wu T, Li S, Zhang Y, Yin X, Yang G, Zhang G.(BioLegend, catalog no. 426102, USA)], and Zombie NIR Fixable Viability Kit (BioLegend, catalog no. 423105, USA). The RedFluor™ 710 Anti-Mouse CD45 (30-F-11) (TONBO Biosciences, catalog no. 80–0451, USA), PerCP-Cyanine5.5 anti-Mouse F4/80 antigen (BM8.1

(8) The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genesNature CommunicationsOctober 17, 2023Donna M. Bond, Oscar Ortega-Recalde, Melanie K. Laird, Takashi Hayakawa, Kyle S. Richardson, Finlay.C. B. Reese, Bruce Kyle, Brooke E. McIsaac-Williams, Bruce C. Robertson, Yolanda van Heezik, et al.for 2 min). The resulting pellet was resuspended in Zombie Near-Infra Red Viability Dye (BioLegend, 423105) and incubated at room temperature for 20 min. The lysate was washed in 5% FBS (in PBS), then resuspended

(9) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate ShuttleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2023She X, Wu Q, Rao Z, Song D, Huang C, Feng S, Liu A, Liu L, Wan K, Li X, et al.min. After washing and centrifugation, the samples were stained with Zombie UV Fixable Viability Kit (423106, BioLegend) to exclude dead cells. Then, the samples were stained with anti‐CD45‐PerCP antibody (103129,

(10) Intrinsic tumor resistance to CAR T cells is a dynamic transcriptional state that is exploitable with low-dose radiationBlood AdvancesSeptember 26, 2023Alexander B. Kim, Ssu-Yu Chou, Solomon Kang, Eric Kwon, Matthew Inkman, Jeff Szymanski, Neal Andruska, Cian Colgan, Jin Zhang, Joanna C. Yang, et al.used to block Fc receptors. In vitro viability after RT was assessed using Zombie NIR (Biolegend cat# 423106) and Annexin V PerCP-Cy5.5 (Biolegend cat# 640936) according to the manufacturer’s protocols. Data were

(11) Sézary syndrome originates from heavily mutated hematopoietic progenitorsBlood AdvancesSeptember 26, 2023Carly M. Harro, Kimberly B. Sprenger, Ricardo A. Chaurio, John J. Powers, Patrick Innamarato, Carmen M. Anadon, Yumeng Zhang, Subir Biswas, Gunjan Mandal, Jessica A. Mine, et al.(CD7-6B7, Cat#343116). Cell viability was assessed through staining with the Zombie NIR (Biolegend, Cat#423106) for 15 minutes in phosphate-buffered saline (PBS). Data were analyzed using FlowJo v10 software. Cell

(12) TRAF4 is crucial for ST2+ memory Th2 cell expansion in IL-33–driven airway inflammationJCI InsightSeptember 22, 2023Jianxin Xiao, Xing Chen, Weiwei Liu, Wen Qian, Katarzyna Bulek, Lingzi Hong, William Miller-Little, Xiaoxia Li, Caini Liudoublets, and then the dead cells were excluded using Zombie NIR Fixable Viability dye (BioLegend, catalog 423105) (Supplemental Figure 1, A and B). The gated live cells were blocked by anti–mouse CD16/32 Ab (clone

(13) Development of new adeno-associated virus capsid variants for targeted gene delivery to human cardiomyocytesMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Cindy Y. Kok, Shinya Tsurusaki, Marti Cabanes-Creus, Sindhu Igoor, Renuka Rao, Rhys Skelton, Sophia H.Y. Liao, Samantha L. Ginn, Maddison Knight, Suzanne Scott, et al.12001-664). Cells were then stained using the Zombie NIR Fixable Viability Kit (BioLegend, San Diego, no. 423105 CA). After washing, cells were fixed in 4% PFA (w/v) for 30 min, then washed and further stained with

(14) Proteomic analysis of signaling pathways modulated by FABP5 in macrophagesResearch SquareSeptember 13, 2023Faniya Doswell, John D. Haley, Martin Kaczochacat. # C10422) at 37°C for 30 min. Cells were also labeled with Zombie staining (Biolegend, cat. # 423105) to quantify viable cells. Cells were harvested and analyzed via flow cytometry by using the mean fluorescence

(15) Topically-applied collagen-binding serum albumin-fused interleukin-4 modulates wound microenvironment in non-healing woundsNPJ Regenerative medicineSeptember 11, 2023Lauterbach AL, Wallace RP, Alpar AT, Refvik KC, Reda JW, Ishihara A, Beckman TN, Slezak AJ, Mizukami Y, Mansurov A, et al.532,58-5920-80,Invitrogen,CXNFT,200 Arg1,PE-Cy7,25-3697-82,Invitrogen,AlexF5,200 Live Dead,Zombie NIR,423105,BioLegend,N/A,800 Flow Cytometric Panel Design for Single Cell Wound Suspension.

(16) Elucidating the direct effects of the novel HDAC inhibitor bocodepsin (OKI-179) on T cells to rationally design regimens for combining with immunotherapyFrontiers in ImmunologySeptember 6, 2023Nisha Holay, Alexander Somma, Mark Duchow, Milad Soleimani, Anna Capasso, Srividya Kottapalli, Joshua Rios, Uma Giri, Jennifer Diamond, Anna Schreiber, et al.evaluated for intracellular proteins were stained with the fixable viability dye Zombie NIR™ (BioLegend 423106) together with surface markers prior to fixation/permeabilization followed by intracellular staining

(17) Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus nonciliated cellsSci AdvAugust 25, 2023Tsz Hong Tsang, Meike Wiese, Martin Helmstädter, Thomas Stehle, Janine Seyfferth, Maria Shvedunova, Herbert Holz, Gerd Walz, Asifa Akhtarresuspended in FACS buffer (0.2% FCS and 2 μM EDTA in PBS) supplemented with 1:500 Zombie-NIR (BioLegend, 423105). For DNA, RNA, or protein analysis, the cells were then passed through a 35-μm cell strainer. GFP-positive

(18) A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathwayCell death & diseaseAugust 24, 2023Wu X, Xu L, Li X, Zhou Y, Han X, Zhang W, Wang W, Guo W, Liu W, Xu Q, et al.expression on the cell membrane. The TILs were stained with the following antibodies: Zombie NIRTM (423105, Biolegend) for dead cell preclusion, CD45-BV510 (103138, Biolegend), CD3-APC (100236, Biolegend), CD4-FITC

(19) Omicron infection following vaccination enhances a broad spectrum of immune responses dependent on infection historyNature communicationsAugust 21, 2023Hornsby H, Nicols AR, Longet S, Liu C, Tomic A, Angyal A, Kronsteiner B, Tyerman JK, Tipton T, Zhang P, et al.re-suspended in the residual volume (~50 μl). Samples were stained with Live/Dead Zombie NIR (Biolegend, 423106) for 10 min at RT, after which and without washing, the surface antibody cocktail diluted in Brilliant Stain Buffer

(20) STING-IRG1 inhibits liver metastasis of colorectal cancer by regulating the polarization of tumor-associated macrophagesiScienceAugust 18, 2023Liu Y, Sun Q, Zhang C, Ding M, Wang C, Zheng Q, Ma Z, Xu H, Zhou G, Wang X, et al.AB_2136290 β-Actin Rabbit mAb,Abclonal,Cat#AC026; RRID: AB_2768234 Zombie NIR™ Fixable Viability Kit,Biolegend,Cat#423105 Brilliant Violet 510™ anti-mouse CD45 Antibody,Biolegend,Cat#103137; RRID: AB_256139 FITC anti-mouse/human CD11b

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(1)

11/29/2023Reliable Zombie NIR™ Fixable Viability Kit From BioLegend Varaang BhandulaThe Zombie NIR viability kit works well for identifying dead cells from cell suspensions isolated from mouse lungs, spleen, and lymph nodes. I recommend storing at -20C to ensure the dye works well ... Read Review
(1)

4/24/2021Relatively Good Fixable Live/Dead Dye Hong SunWe are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.
(1)

1/11/2021Using Zombie NIR to Detect Dead Cells Alejandro PradosWe are using the Zombie NIR to characterize the immune cells populations in the mouse intestine during chronic inflammation. This reagent is perfect to clearly separate dead and live cells.
(1)

3/1/2016Cell Viability Kit for FC Maria Carmela SperanzaThis is a good and easy to use cell viability staining kit for flow cytometry.

Zombie NIR(TM) Fixable Viability Kit from BioLegend

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Zombie NIR(TM) Fixable Viability Kit from BioLegend

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