Zombie Aqua(TM) Fixable Viability Kit 423101 from BioLegend

Product Specs
ItemZombie Aqua(TM) Fixable Viability Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423101
Quantity100 tests
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) AMPK activation induces immunogenic cell death in AMLBlood AdvancesDecember 26, 2023Johanna Mondesir, Margherita Ghisi, Laura Poillet, Robert A. Bossong, Oliver Kepp, Guido Kroemer, Jean-Emmanuel Sarry, Jérôme Tamburini, Andrew A. Lane(Cell Signaling Technology, no. 4083S); Zombie Green (BioLegend, no. 423111); Zombie Aqua (BioLegend, no. 423101); anti-CALR antibody, ER marker (Abcam, no. ab 2907); goat anti-rabbit IgG (H+L), secondary antibody

(2) Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironmentNat CommunDecember 20, 2023Xiaojian Lu, Yanwei Luo, Xichen Nie, Bailing Zhang, Xiaoyan Wang, Ran Li, Guangmin Liu, Qianyin Zhou, Zhizhong Liu, Liqing Fan, et al.previously reported using the flow cytometer (Cytec, USA)74,77. Zombie Aqua fixable viability kit (catalog: 423101, 1:200), APC-anti-human CD19 (catalog: 302212, 1:100), PE-Cy7-anti-human 4-1BB (catalog: 309817, 1:100)

(3) An epitope encoded by uORF of RNF10 elicits a therapeutic anti-tumor immune responseMolecular Therapy - OncolyticsDecember 19, 2023Lili Zeng, Wei Zheng, Jiahui Zhang, Jiawen Wang, Qing Ji, Xinglong Wu, Yaming Meng, Xiaofeng Zhuwere incubated for 5 h at 37°C, stained with Zombie Aqua Fixable Viability Kit (BV525, BioLegend, Cat# 423101), anti-mouse CD8 (PerCP, BioLegend, Cat# 100732), and anti-mouse CD3 (APC-Cy 7, BD Pharmingen, Cat#

(4) Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicityFront ImmunolDecember 8, 2023Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin, et al.Fixable Viability Kit (Biolegend, Cat.: 423105) Zombie Aqua™ Fixable Viability Kit (Biolegend, Cat.: 423101), fixable viability dye e780 (eBioscience, Cat.: 65-0865) or the BD Horizon™ Fixable Viability Stain 700

(5) Innate and Adaptive Cell-Mediated Immune Responses to a COVID-19 mRNA Vaccine in Young ChildrenOpen Forum Infect DisDecember 2, 2023Adriana Weinberg, Michael J Johnson, Krystle Garth, Elena W Y Hsieh, Ross Kedl, Daniela Weiskopf, Mattie Cassaday, Cody Rester, Berenice Cabrera-Martinez, Ryan M Baxter, et al.incubation at 37 °C in a humidified 5% CO2 atmosphere, cells were stained with zombie aqua (Biolegend 423101) viability dye and monoclonal antibodies recognizing the following cell surface markers: CD19 (BV605,

(6) Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysisSTAR ProtocolsDecember 1, 2023Enitome E. Bafor, Toni Martin, Jeffrey Carrell, Megan Karwan, Adrienne E. Kimmel, Uyi.G. Omogiade, Michael Sanford, Howard A. Young, Julio C. Valencia90 μL of FACS buffer. Use immediately.•Viability dye (Zombie Aqua Fixable Viability Kit, BioLegend #423102).•RPMI medium.CRITICAL: Prepare FBS under sterile conditions. Filter the solution through a 75 mm rapid

(7) Iron overload induces cerebral endothelial senescence in aged mice and in primary culture in a sex‐dependent mannerAging CellNovember 1, 2023Brian Noh, Maria Pilar Blasco‐Conesa, Syed Mushfiqur Rahman, Sheelu Monga, Rodney Ritzel, Gary Guzman, Yun‐Ju Lai, Bhanu Priya Ganesh, Akihiko Urayama, Louise D. McCullough, et al.#124010). The fixable viability dye Zombie Aqua was used for live/dead discrimination (Biolegend, #423102). Cells were then washed in FACS buffer, fixed in 2% paraformaldehyde for 8 m, and washed once more

(8) PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease modelsThe Journal of clinical investigationNovember 1, 2023Baldrighi M, Doreth C, Li Y, Zhao X, Warner E, Chenoweth H, Kishore K, Umrania Y, Minde DP, Thome S, et al.counter (Chemometec). Collected cell samples were then stained with Zombie Aqua viability dye (BioLegend, 423102) for 10 minutes at room temperature in the dark. Then, samples were stained with the desired antibodies

(9) Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8+ T cellsNature CommunicationsOctober 25, 2023Diego Calzada-Fraile, Salvador Iborra, Marta Ramírez-Huesca, Inmaculada Jorge, Enrico Dotta, Elena Hernández-García, Noa Martín-Cófreces, Estanislao Nistal-Villán, Esteban Veiga, Jesús Vázquez, et al.for flow cytometry. In this case, the live/dead probe used was Zombie Aqua Fixable Viability Kit (Cat# 423101, BioLegend). For biotin detection, an anti-biotin PE antibody (in MHC-I) or Streptavidin-BV421 (in lipid

(10) Intravenous BCG vaccination reduces SARS-CoV-2 severity and promotes extensive reprogramming of lung immune cellsiScienceOctober 20, 2023Singh AK, Wang R, Lombardo KA, Praharaj M, Bullen CK, Um P, Gupta M, Srikrishna G, Davis S, Komm O, et al.were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Biolegend; 423101) for 20 min at room temperature. Cells were subsequently washed and resuspended in FACS buffer (1% BSA,

(11) Oncolytic Viral Therapy for Glioma by Recombinant Sindbis VirusCancers (Basel)September 27, 2023Kangyixin Sun, Xiangwei Shi, Li Li, Xiupeng Nie, Lin Xu, Fan Jia, Fuqiang Xumin. Cells were resuspended by adding 200 µL PBS, and 1 µL Zombie Aqua™ Fixable Viability Kit (Cat# 423101, Biolegend, San Diego, CA, USA) was added to exclude dead cells, incubated on ice for 3–5 min, and then

(12) Alleviating psoriatic skin inflammation through augmentation of Treg cells via CTLA-4 signaling peptideFrontiers in ImmunologySeptember 25, 2023Woo-Sung Lee, Kyung-Ho Nam, Jong Hoon Kim, Won-Ju Kim, Jeong Eun Kim, Eui-Cheol Shin, Gil-Ran Kim, Je-Min Choi4 h at 37°C. To exclude dead cells, cells were stained with the Zombie Aqua Fixable Viability Kit (423101; BioLegend) at room temperature for 10 min. After washing with PBS, the surface proteins were stained

(13) Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid CellsBIO-PROTOCOLSeptember 20, 2023Pauline Loos, Thomas Marichal, Bénédicte Machiels, Laurent Gillet)Recombinant murine IL-2 (Peprotech, catalog number: 212-12)Zombie Aqua Fixable Viability kit (BioLegend, catalog number: 423101)Sytox Blue Dead Cell stain (Thermo Fisher, catalog number: s34857)Anti-mouse CD16/32 Fc block clone

(14) Assessing the role of T cells in response to retinal injury to uncover new therapeutic targets for the treatment of retinal degenerationJournal of NeuroinflammationSeptember 9, 2023Federica M. Conedera, Judith M. Runnels, Jens V. Stein, Clemens Alt, Volker Enzmann, Charles P. Linwere stained with the following fluorescently labeled markers: Zombie Aqua™ Fixable Viability Kit (423101; Biolegend), FITC anti-mouse CD45 antibody (103108; Biolegend), APC/Cyanine7 anti-mouse CD3 antibody

(15) Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen SpeciesPathogensSeptember 9, 2023Kayla M. Fantone, Joanna B. Goldberg, Arlene A. Stecenko, Balázs RadaMRSA24, PMNs were stained with Zombie Aqua™ Fixable Viability kit (Biolegend, San Diego, CA, USA cat#423102) at a dilution of 1:500 for 15 min after washing following infection. PMNs were then washed and resuspended

(16) Inhibition of TCA cycle improves the anti-PD-1 immunotherapy efficacy in melanoma cells via ATF3-mediated PD-L1 expression and glycolysisJournal for ImmunoTherapy of CancerSeptember 7, 2023Nian Liu, Mingjie Yan, Qian Tao, Jie Wu, Jing Chen, Xiang Chen, Cong Pengas single cell suspensions, stained with Zombie Aqua Fixable Viability Kit (anti-BV510, BioLegend, #423102, USA), incubated for 15 min at room temperature, and then terminated and centrifuged. The fragment crystallizable

(17) Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brainJournal of neuroinflammationAugust 31, 2023Li Y, Khan N, Ritzel RM, Lei Z, Allen S, Faden AI, Wu J.leukocyte scatter properties, singlets, and exclusion of the fixable viability dye Zombie Aqua (Cat# 423102, Biolegend). Living cells were identified as microglia (CD45intCD11b+) and infiltrating myeloid cells

(18) Rescue of Alzheimer's disease phenotype in a mouse model by transplantation of wild-type hematopoietic stem and progenitor cellsCell reportsAugust 29, 2023Mishra P, Silva A, Sharma J, Nguyen J, Pizzo DP, Hinz D, Sahoo D, Cherqui S.anti-CD11b-PE (101207, Biolegend), and Zombie Aqua Fixable Viability Kit were used for live cells staining (423101, Biolegend). Data were acquired on an Aria II and Aria fusion (BD Bioscience) and FlowJo software (BD

(19) Small Heterodimer Partner Modulates Macrophage Differentiation during Innate Immune Response through the Regulation of Peroxisome Proliferator Activated Receptor Gamma, Mitogen-Activated Protein Kinase, and Nuclear Factor Kappa B PathwaysBiomedicinesAugust 28, 2023Forkan Ahamed, Natalie Eppler, Elizabeth Jones, Lily He, Yuxia Zhang(BioLegend, USA, 128017) anti-mouse antibodies, and the fixable viability dye (Zombie Aqua, BioLegend, 423101) for 30 min on ice. After centrifugation (300× g) for 5 min, the cells were washed twice with 1 mL of

(20) Immunotherapy targeting different immune compartments in combination with radiation therapy induces regression of resistant tumorsNat CommunAugust 24, 2023Nils-Petter Rudqvist, Maud Charpentier, Claire Lhuillier, Erik Wennerberg, Sheila Spada, Caroline Sheridan, Xi Kathy Zhou, Tuo Zhang, Silvia C. Formenti, Jennifer S. Sims, et al.incubated on ice for 30 min and washed in PBS. Viability dye staining (Zombie Aqua, Biolegend cat #423101 or Zombie UV, Biolegend cat #423107) was performed prior to surface staining with the following antibodies:

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua(TM) Fixable Viability Kit from BioLegend

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Zombie Aqua(TM) Fixable Viability Kit from BioLegend

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