Annexin V-FITC Early Apoptosis Detection Kit 6592S from Cell Signaling Technology

Product Specs
ItemAnnexin V-FITC Early Apoptosis Detection Kit
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number6592S
Quantity1 Kit
Conjugate/Tag/LabelFITC
Detection TargetANXA5
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Research AreaRelated Products
ApplicationsIF-IC, FC-L
ReactivityAll

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
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Website: http://www.cellsignal.com/

Website: Technical Support

(1) FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR SignalingCellsJuly 29, 2023Jun Zhang, Xin Li, Yunfei Zhou, Mingming Lin, Qianying Zhang, Yunhong WangScientific (Waltham, MA, USA). The Annexin V-FITC early apoptosis detection kit was obtained from CST (#6592) ( Danvers, MA, USA). The basement membrane extract (BME) was obtained from TianGEN (Matrigel®) (Shanghai,

(2) Trans-differentiation of trophoblast stem cells: implications in placental biologyLife Science AllianceMarch 1, 2023Madhurima Paul, Shreeta Chakraborty, Safirul Islam, Rupasri Ain-cultured cells were processed either for both annexin V–PI staining using early apoptosis detection kit (cat no. 6592; Cell Signaling Technology) by flow cytometry or for protein isolation and Western blotting for detection

(3) Ladinin 1 Shortens Survival via Promoting Proliferation and Enhancing Invasiveness in Lung AdenocarcinomaInternational Journal of Molecular SciencesDecember 27, 2022Chao-Yuan Chang, Yung-Chi Huang, Hung-Hsing Chiang, Yu-Yuan Wu, Kuan-Li Wu, Yung-Yun Chang, Lian-Xiu Liu, Ying-Ming Tsai, Ya-Ling Hsuthen processed to stain with Annexin V-FITC Early Apoptosis Detection Kit (Cell signaling technology, #6592) according to the manufacturer’s instructions. The flow cytometry analysis was performed by BD Accuri™ C6 Plus Flow Cytometer

(4) The Microalga Skeletonema marinoi Induces Apoptosis and DNA Damage in K562 Cell Line by Modulating NADPH OxidaseMoleculesNovember 27, 2022Roberto Ciarcia, Consiglia Longobardi, Gianmarco Ferrara, Serena Montagnaro, Emanuela Andretta, Francesco Pagnini, Salvatore Florio, Lucianna Maruccio, Chiara Lauritano, Sara Damianoapoptotic cells. A Cell Signaling Annexin V-FITC kit (Annexin V-FITC Early Apoptosis Detection Kit #6592) was used as described by the manufacturer. Briefly, after the incubation, cells were collected by centrifugation

(5) Leveraging patient derived models of FGFR2 fusion positive intrahepatic cholangiocarcinoma to identify synergistic therapiesnpj Precision OncologyOctober 23, 2022Michael E. Lidsky, Zechen Wang, Min Lu, Annie Liu, S. David Hsu, Shannon J. McCall, Zhecheng Sheng, Joshua A. Granek, Kouros Owzar, Karen S. Anderson, et al.supplied with 1% HI-FBS. Cells were stained with Annexin V-FITC early apoptosis detection kit (CST, Cat# 6592) according to the manufacturer’s protocol. Organoids were split 1:5–1:10 in 24-well plates and cultured

(6) Generation of functional human oligodendrocytes from dermal fibroblasts by direct lineage conversionDevelopmentOctober 15, 2022Koji Tanabe, Hiroko Nobuta, Nan Yang, Cheen Euong Ang, Philip Huie, Sacha Jordan, Michael C. Oldham, David H. Rowitch, Marius Wernig(AbD Serotec, MCA409S, 1:1000); STEM121 (Takara Bio, Y40410, 1:1000); AnnexinV (Cell Signaling Technology, 6592S, staining protocol provided by manufacturer); CD13 (BD Biosciences, 557454, 1:100). Secondary antibodies

(7) An immunogenic cell injury module for the single-cell multiplexed activity metabolomics platform to identify promising anti-cancer natural productsThe Journal of biological chemistrySeptember 1, 2022Balsamo JA, Penton KE, Zhao Z, Hayes MJ, Lima SM, Irish JM, Bachmann BO.were not washed prior to live cell data acquisition consistent with manufacturer’s instructions (CST 6592). DAPI was used to replace PI. Profiling: cell culture K562, Jurkat, and MV-4-11 cell lines were a

(8) DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial DysfunctionInternational journal of molecular sciencesMarch 6, 2022Le AN, Park SS, Le MX, Lee UH, Ko BK, Lim HR, Yu R, Choi SH, Lee BJ, Ham SY, et al.manufacturer. 4.12. Annexin-V Staining Apoptotic cells were stained using an Annexin-V-FLUOS staining kit (#6592, Cell Signaling), according to the protocol supplied by the manufacturer. Annexin-V-stained cells were

(9) LDLR dysfunction induces LDL accumulation and promotes pulmonary fibrosisClinical and translational medicineJanuary 1, 2022Shi X, Chen Y, Liu Q, Mei X, Liu J, Tang Y, Luo R, Sun D, Ma Y, Wu W, et al.assays. The Sircol Assay Kit (S5000, Biocolor), Annexin V‐Alexa Fluor 488/PI Apoptosis Detection Kit (6592, CST, Massachusetts, USA), Endothelin 1 ELISA Kit (DET100, R&D), TUNEL Kit (11684795910, Roche, Indianapolis,

(10) Glucose transporter‑1 inhibition overcomes imatinib resistance in gastrointestinal stromal tumor cellsOncology reportsJanuary 1, 2022Shima T, Taniguchi K, Tokumaru Y, Inomata Y, Arima J, Lee SW, Takabe K, Yoshida K, Uchiyama K.V-FITC/PI staining on 1×106 cells using the Annexin V-FITC Early Apoptosis Detection Kit (cat. no. 6592; Cell Signaling Technology, Inc.). To 1×106 cells, 1 µl Annexin V-FITC and 12.5 µl PI were added and

(11) Anti-apoptotic effect of adipose tissue-derived stromal vascular fraction in denervated rat muscleStem Cell Research & TherapyDecember 1, 2021R. El-Habta, G. Andersson, P. J. Kingham, L. J. Backmanapoptotic cells were identified using Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling; #6592) following the instructions provided by the manufacturer. In brief, cells were collected by centrifugation,

(12) Two parallel pathways connect glutamine metabolism and mTORC1 activity to regulate glutamoptosisNature CommunicationsDecember 1, 2021Clément Bodineau et al.stained following with annexin V and propidium iodide (PI) (Annexin V early apoptosis detection kit, #6592 Cell Signaling Technology) according to the manufacturer’s instructions. Finally, cells were analysed

(13) Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 SignalingFront Endocrinol (Lausanne)July 5, 2021Hui-Hui Wang, Jia-Ni Ma, Xiao-Rong Zhanstained with Annexin V-FITC and PI staining reagents under the instruction of detection kit (CST, USA, #6592) in dark condition for 20 minutes. Afterward, the cells were washed and resuspended in binding buffer

(14) Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling in vivo and in vitroInternational Journal of Molecular MedicineJuly 2, 2021Cong Cong, Xiaohong Yuan, Ying Hu, Wenjing Chen, Yong Wang, Lei Taoplated in 6-well plates. Cell apoptosis was assessed using the Annexin V-FITC Apoptosis Detection kit (#6592, Cell Signaling Technology, Inc.) according to the manufacturer's instructions. In brief, ~2×106 cells

(15) Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem CellsEnvironmental health perspectivesJuly 1, 2021Li B, Qiao C, Jin X, Chan HM.Apoptosis The Annexin V–fluorescein-5-isothiocyanate (FITC) Early Apoptosis Detection Kit (Catalog no. 6592S; Cell Signaling Technology) was used according to the manufacturer’s instructions to detect the effects

(16) Laurocerasus officinalis Roem. fruit extract induces cell death through caspase mediated apoptosis in gastric cancer cell linesTurkish Journal of BiochemistryApril 27, 2021Nihal Karakaş, Mehmet Evren Okur, Nurşah Öztunç, Derya Çiçek Polat, Ayşe Esra Karadağearly apoptosis double staining protocol was applied according to manufacturer’s instructions (CST #6592 Annexin V-FITC Early Apoptosis Kit). Then, early apoptotic, late apoptotic, necrotic, and live cell

(17) Discovery of a novel ferroptosis inducer-talaroconvolutin A-killing colorectal cancer cells in vitro and in vivoCell death & diseaseNovember 17, 2020Xia Y, Liu S, Li C, Ai Z, Shen W, Ren W, Yang X.were harvested by trypsin treatment, and stained by Annexin V-FITC Early Apoptosis Detection Kit (#6592, Cell Signaling Technology, USA) for 20 min in darkness. The stained cells were counted and recorded

(18) DSTYK Promotes Metastasis and Chemoresistance via EMT in Colorectal CancerFrontiers in PharmacologySeptember 2, 2020Jinyu Zhang, Zachary Miller, Phillip R. Musich, Ashlin E. Thomas, Zhi Q. Yao, Qian Xie, Philip H. Howe, Yong Jiangstaining with Annexin V-FITC and PI according to the specified protocol (Cell Signaling Technology, cat# 6592). Briefly, cells were resuspended in 500 µl 1× binding buffer containing 5 µl Annexin V-fluorescein

(19) Isoliquiritigenin ameliorates caerulein-induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophagesJournal of cellular and molecular medicineSeptember 1, 2020Wang LJ, He L, Hao L, Guo HL, Zeng XP, Bi YW, Lu GT, Li ZS, Hu LH.(Na‐CMC) was obtained from Sangon Biotechnology (Shanghai, China). Early Apoptosis Detection Kit (#6592) was obtained from Cell Signaling Technology (Danvers, Massachusetts, USA). 2.2 Mice C57BL/6 male

(20) SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR-23b-3p via sponging EGR1 in hepatocellular carcinomaCancer medicineJune 1, 2020Jing Z, Ye X, Ma X, Hu X, Yang W, Shi J, Chen G, Gong L.transfected Hep3B, or Hep3B/So cells were determined by Annexin V‐FITC Early Apoptosis Detection Kit (#6592; Cell Signaling). Hep3B cells were suspended in 500 µL binding buffer and then incubated at room temperature.

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Annexin V-FITC Early Apoptosis Detection Kit

Annexin V-FITC Early Apoptosis Detection Kit from Cell Signaling Technology

Cell Signaling Technology

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Annexin V-FITC Early Apoptosis Detection Kit from Cell Signaling Technology

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