Human Follicular dendritic cell secreted peptide, C4orf7 ELISA Kit from MyBioSource.com

Description

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4orf7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4orf7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4orf7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4orf7 bound in the initial step. The color development is stopped and the intensity of the color is measured